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flipper tr er specific  (Spirochrome)


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    Spirochrome flipper tr er specific
    Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 <t>from</t> <t>Flipper-TR</t> (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.
    Flipper Tr Er Specific, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flipper tr er specific/product/Spirochrome
    Average 94 stars, based on 13 article reviews
    flipper tr er specific - by Bioz Stars, 2026-03
    94/100 stars

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    1) Product Images from "Calcium waves and nuclear tension changes coordinate mechanical stress dissipation in locally folded epithelia"

    Article Title: Calcium waves and nuclear tension changes coordinate mechanical stress dissipation in locally folded epithelia

    Journal: iScience

    doi: 10.1016/j.isci.2025.114477

    Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 from Flipper-TR (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.
    Figure Legend Snippet: Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 from Flipper-TR (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.

    Techniques Used: Phospho-proteomics, Western Blot, MANN-WHITNEY, Staining, Clinical Proteomics, Membrane, Control, Over Expression



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    flipper tr er specific  (Spirochrome)
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    Spirochrome flipper tr er specific
    Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 <t>from</t> <t>Flipper-TR</t> (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.
    Flipper Tr Er Specific, supplied by Spirochrome, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flipper tr er specific/product/Spirochrome
    Average 94 stars, based on 1 article reviews
    flipper tr er specific - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    flipper tr er specific spirochrome  (Spirochrome)
    96
    Spirochrome flipper tr er specific spirochrome
    Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 <t>from</t> <t>Flipper-TR</t> (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.
    Flipper Tr Er Specific Spirochrome, supplied by Spirochrome, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flipper tr er specific spirochrome/product/Spirochrome
    Average 96 stars, based on 1 article reviews
    flipper tr er specific spirochrome - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

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    Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 from Flipper-TR (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.

    Journal: iScience

    Article Title: Calcium waves and nuclear tension changes coordinate mechanical stress dissipation in locally folded epithelia

    doi: 10.1016/j.isci.2025.114477

    Figure Lengend Snippet: Level of nuclear envelope tension is essential to trigger nuclear recovery upon epithelial folding (A) Changes in nuclear area upon folding (violet) of confluent monolayers (CM) and highly confluent monolayers (HCM). Data are represented as mean ± SEM, N = 3 independent experiments. (B) Quantification of connexin43 phosphorylation state (Cx43/pCx43) based on the Western blot experiments. Each point represents a separate replicate for CM and HCM; Data are represented as mean ± SEM; n.s. - no statistically significant, Mann-Whitney. (C) Quantification of nuclear area change between CM and HCM (∗∗ p = 0,0079, Mann-Whitney, n > 20 nuclei per condition, N = 2 replicates) and corresponding fluorescent images of CM and HCM stained with anti-Lamin A/C antibody; scale bars: 10 μm. (D) Quantification of τ2 from Flipper-TR (plasma membrane) and ER Flipper-TR (nuclear membrane) stained cells using FLIM; n > 100 cells/condition pooled across three independent experiments; ∗∗∗∗ p < 0.0001, Mann-Whitney; Data are represented as mean ± SEM. (E) Fluorescent images with anti-Lamin A/C staining of control and cells with LBR-RFP overexpression; Scale bars 5 μm. (F) Western blot of control and cells overexpressing LBR-RFP with anti-RFP antibody. (G) Quantification of LBR-RFP overexpression based on the Western blot from F. Each point represents an independent experiment. ∗∗∗∗ p < 0.0001 Mann-Whitney test was used. Data are represented as mean ± SEM. (H) Quantification of τ2 from ER Flipper-TR (nuclear membrane) of control cells and LBR-RFP cells. N = 3 Independent experiments (with n = 140 measurements per condition); data are pooled together. ∗∗∗∗ p < 0.0001, t test was used; Data are represented as mean ± SEM. (I) Normalized intensity profile of calcium wave (Fluo4AM) as a function of distance at t = T max = 18 s for control and LBR-RFP cells, and the corresponding images; data are represented as mean ± SEM; Scale bars 50 μm. (J) Nuclear area changes of LBR-RFP cells upon folding in the function of time; Data are represented as mean ± SEM. N = 3 Independent experiments. Statistical analysis of the change of area at the t stretch and t stretch+250 s can be found in Supplementary Figures. (K) Images of a single nucleus of LBR-RFP cells marked with Hoechst at three time points: before the stretch (t = 6 s), stretched (t = 15 s), and stretched +300 s. The color masks of the nucleus correspond to different time points and come from the image analysis processing. Scale bar 10 μm.

    Article Snippet: Flipper-TR ER specific , Spirochrome , #SC021.

    Techniques: Phospho-proteomics, Western Blot, MANN-WHITNEY, Staining, Clinical Proteomics, Membrane, Control, Over Expression